In Vitro Labeling of RNA in Isolated Nucleoli of the Walker Tumor and Liver* TAB SUK Ro AND HARRIS BUSCH
ثبت نشده
چکیده
ethanol was also required for the optimal activity. The pH optimum was 8.2 for both tumor and liver nucleoli. Maximum incorporation of isotope into RNA was found at 15-30 minutes after the incubation was begun. The reactions were DNA-dependent, as shown by sensitivity to actinomycin D. Evidence for internucleotide incorporation of ATP-P32 was obtained by analysis of P32distribution into nucleotide monophos-
منابع مشابه
a2p-Distribution in Oligonucleotides of Rapidly Sedimenting Nucleolar RNA's of Hepatomas and Normal Rat Liver
Nucleolar 28 S, 35 S, and 45 S RNA's were isolated from normal rat liver, Novikoff hepatoma ascites cells, and Morris hepatoma 9618A after short (60 min) and long (6 hr) labeling with orthophosphate-32p. After purification by sucrose density sedimentation, the 32p nucleotide composition of these RNA's was determined. The RNA was hydrolyzed to oligonucleotides with pancreatic ribonuclease. The d...
متن کاملبررسی آزمایشگاهی بیماری زایی قارچ Verticillium lecanii (Zimm.) Viegas روی شته نخود فرنگی [Acyrthosiphon Pisum (Harris)]
Pathogenicity and virulence of entomogenous fungus, Verticillium lecanii, was studied on the pea aphid, Acyrthosiphon pisum. Vertalec, a commercial product of V. lecanii, was evaluated under controlled conditions. Second instar nymphs were inoculated with conidial suspensions at concentrations ranging from 104 to 108 conidia/ml, and sterile distilled water (containing wetting agent) as control,...
متن کاملبررسی آزمایشگاهی بیماری زایی قارچ Verticillium lecanii (Zimm.) Viegas روی شته نخود فرنگی [Acyrthosiphon Pisum (Harris)]
Pathogenicity and virulence of entomogenous fungus, Verticillium lecanii, was studied on the pea aphid, Acyrthosiphon pisum. Vertalec, a commercial product of V. lecanii, was evaluated under controlled conditions. Second instar nymphs were inoculated with conidial suspensions at concentrations ranging from 104 to 108 conidia/ml, and sterile distilled water (containing wetting agent) as control,...
متن کاملTricuspid valve erosion from Swan-Ganz catheters.
CHEST, 80: 4, OCTOBER, 1981 TRICUSPID VALVE EROSION FROM SWAN-GANZ CATHETERS 509 monaistenosen durch tumourkonipression. Wien Kiln Wochenschr 1979; 91:429 5 Harris M, Woo-Ming MO, Miller CC. Acquired pulmonary stenosis due to compression by a bronchiogenic cyst. Thorax 1973; 28:394 6 Son R, Nakhjavan FK, Goldberg H. Mediastinal tumor simulating idiopathic dilation of pulmonary artery: diagnosis...
متن کاملSome Observations Co Ncerning
One of the more perplexing aspects of mitosis has been the na ture of the mechan ism whereby the chromosomes are moved into their metaphase configuration. Early theories of chromosome movemen t which involved the spindle or chromosomal fibers later gave way to theories proposing alternate mechanisms, as spindle fibers came to be regarded as artifacts of fixation. In recent years, however, evide...
متن کامل